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GrainGenes Sequence Report: Ta.11623.1.S1_x_at

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Sequence	BQ806858
Contig	Ta.11623.1.S1_s_at Ta.11623.1.S1_x_at NSFT03P2_Contig10696
External Databases	Data at GenBank Data at EMBL Data at DDBJ
NCBI UniGene	Ta.28720
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, moderately similar to NP_001062132.1 Os08g0495500 [Oryza sativa (japonica cultivar-group)]'
Keyword	EST
Species	Triticum aestivum
Cultivar	Butte 86
Clone Library	Wheat developing grains cDNA library
Tissue	whole grains
Developmental Stage	3-44 days post anthesis seed
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE3584_A03_A06ZS Wheat developing grains cDNA library Triticum aestivum cDNA clone WHE3584_A03_A06, mRNA sequence.
Strain	lab_host E. coli SOLR
Clone	WHE3584_A03_A06
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: SK primer. Note: Vector: Lambda ZAP II, excised phagemid; Site_1: EcoRI; Plants were grown under six following different environmental regimes in greenhouse, Environment 1) 24oC/17oC day/night, well-watered, with post-anthesis fertilizer, Environment 2) 24oC/17oC day/night, well-watered, without post-anthesis fertilizer, Environment 3) 37oC/17oC day/night, well-watered, with post-anthesis fertilizer, Environment 4) 37oC/17oC day/night, well-watered, without post-anthesis fertilizer, Environment 5) 37oC/17oC day/night plus drought, with post-anthesis fertilizer, Environment 6) 37oC/17oC day/night plus drought, without post-anthesis fertilizer, developing wheat grains from the following were excised and frozen in liquid nitrogen, Environment 1 at 3, 5, 7, 8, 10, 12, 16, 20, 24, 28, 32, 36, 40, 44 DPA Environment 2 at 3, 5, 7, 8, 10, 12, 16, 20, 24, 28, 32, 36, 40, 44 DPA Environment 3 at 3, 5, 7, 8, 10, 12, 16, 20, 24, 28, 32, 34 DPA Environment 4 at 3, 5, 7, 8, 10, 12, 16, 20, 24 , 28, 32, 34 DPA Environment 5 at 3, 5, 7, 8, 10, 12, 16, 20, 24, 28, 30 DPA Environment 6 at 3, 5, 7, 8, 10, 12, 16 , 20, 24, 28, 30 DPA and total RNA was prepared by S. Altenbach and K. Cronin at USDA-ARS, Albany, CA. A cDNA library was made using poly (A) RNA, and the cDNA clones were in vivo excised to give pBluescript SK(-) phagemids in the TJ Close lab (Chin, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (others). Note: Vector: Lambda ZAP II, excised phagemid; Site_1: EcoRI; Plants were grown under six following different environmental regimes in greenhouse, Environment 1) 24oC/17oC day/night, well-watered, with post-anthesis fertilizer, Environment 2) 24oC/17oC day/night, well-watered, without post-anthesis fertilizer, Environment 3) 37oC/17oC day/night, well-watered, with post-anthesis fertilizer, Environment 4) 37oC/17oC day/night, well-watered, without post-anthesis fertilizer, Environment 5) 37oC/17oC day/night plus drought, with post-anthesis fertilizer, Environment 6) 37oC/17oC day/night plus drought, without post-anthesis fertilizer, developing wheat grains from the following were excised and frozen in liquid nitrogen, Environment 1 at 3, 5, 7, 8, 10, 12, 16, 20, 24, 28, 32, 36, 40, 44 DPA Environment 2 at 3, 5, 7, 8, 10, 12, 16, 20, 24, 28, 32, 36, 40, 44 DPA Environment 3 at 3, 5, 7, 8, 10, 12, 16, 20, 24, 28, 32, 34 DPA Environment 4 at 3, 5, 7, 8, 10, 12, 16, 20, 24, 28, 32, 34 DPA Environment 5 at 3, 5, 7, 8, 10, 12, 16, 20, 24, 28, 30 DPA Environment 6 at 3, 5, 7, 8, 10, 12, 16, 20, 24, 28, 30 DPA and total RNA was prepared by S. Altenbach and K. Cronin at USDA-ARS, Albany, CA. A cDNA library was made using poly (A) RNA, and the cDNA clones were in vivo excised to give pBluescript SK(-) phagemids in the TJ Close lab (Chin, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (others).
DNA	tcggcacgaggctccctcccacagtcccactcccatgtcctctgccatcc catccccatcccaccaccatatagccgccactccacccccacccccaccc cacctccttccgcgcgcgccgctccccaagaaccgggacggagagctagc cctccgcgactccctcgccgcgcgcacgcttcctgcatgccgctgtgacc gtgagtcgtcgtcgtactgatctaccccgccatgtcgtcgtgctgagtgg tgggtgggcgttcggggtctggccgtgcgcgcgcgggttggcggcagtac atgaggaggagggccgggaggagggagcctggggaagggggcgctggggc tgctgggctgcgtcgatgaggtggtggcggcggtggcgtcgtcgtggggt gcgatgagcgccgcggggtcccgggtcgaggcggcgccgcggctcgcgca atggcgcgtcgacgccctcccctgctacacctaccgcaagtccgactcct tccgcgtcggcctctggaactggtacctgtctgtggagagaaacaacaag caaacttgcgttaagctctttgcagagttgtcaaattctgccaagaacac agcccccgcaccgatagcctcgtttgtcacgaaacttctgatatcctttc ctccagatcagaagatcatagagcatccaggtatcctggacaagcctctg aaac

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