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GrainGenes Sequence Report: NSFT03P2_Contig8061

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Sequence	BQ789057
Contig	Ta.4425.1.S1_at NSFT03P2_Contig8061
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC322827
NCBI UniGene	Ta.47512
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Clone wlmk8.pk0018.e8:fis, full insert mRNA sequence'
Keyword	EST
Species	Triticum aestivum
Cultivar	Chinese Spring
Clone Library	Wheat CS whole plant cDNA library
Tissue	Roots, leaves, crown, stem and sheath
Developmental Stage	Adult
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE4157_A10_B19ZS Wheat CS whole plant cDNA library Triticum aestivum cDNA clone WHE4157_A10_B19, mRNA sequence.
Strain	lab_host E. coli SOLR
Clone	WHE4157_A10_B19
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid pBluescript SK(-); Site_1: EcoRI; Site_2: XhoI; Plant tissues from wheat cv. CS grown to full tillering stage in greenhouse were collected at University of California, Davis (Jan Dvorak's lab, K. Deal and E. Akhunov). Total RNA was prepared from leaves (young leaf and third leaf), whole roots, crown, steam and sheath tissues, and then equal quantities of RNA were pooled from the these samples. PolyA was purified from the pooled RNA, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript SK(-) phagemids in J. Dvorak's lab (E. Akhunov, J. Dvorak) at the University of California, Davis. Colony plating, plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA	gaggcggaggcgctcccgcgggtgcggctcgcggccgtcgacgtggagtg ggcgcacgtgctcgccgagggctgggcgtcgccgctgcgcgggttcatgc gggagcacgagtacctccagtgcctccacttcaactccctccgcctcccc tccggcggcctcgccaacatgtcgctccccatcgtgctcgccgtcgacga cgccgccaaggaccgcgtcggcgccgcccccgacgtcgcgctcgccgggc ccgacggcgagctcctcgccgtcctccgcagtgtcgaaatataccctcac aataaagaagaaaggattgcaagaacatgggggacaactgcgcctggctt accttatgtcgatgaggcgataacaccagctggaaactggctgattggtg gtgatctggaggtgttgcaacccattaagtataacgatggccttgaccat tacaggctttcaccccagcaacttagggacgaattcgacaagcgtggggc tgatgctgtatttgcattccagttgagaaacccagtccacaatgggcatg cactgttgatgaatgacactagaaggcgtctcttggaaatgggtttcaag aatcccattctactgctacagccccttgggtggttttacaaaagctgatg atgtcccgctgcctgttagaatggaacaacacagcaaggtcttagaagat ggagtccttgaccccgagaccactatcgtgtctatatttccctccccaat gcattatgctggtccagcagaagtgcagtggcatgcaaag

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