GrainGenes Sequence Report: BQ753146

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Sequence

BQ753146

External Databases

Data at GenBank

Data at EMBL

Data at DDBJ

DB Remark

Locus Source: Triticum aestivum (bread wheat)

Keyword

EST

Species

Triticum aestivum

Cultivar

Chinese Spring

Clone Library

Wheat salt-stressed root cDNA library

Tissue

Roots

Developmental Stage

Full tillering

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

WHE4123_E11_J21ZS Wheat salt-stressed root cDNA library Triticum aestivum cDNA clone WHE4123_E11_J21, mRNA sequence.

Strain

lab_host E. coli SOLR

Clone

WHE4123_E11_J21

Probe

MAG449

MAG633

MAG1570

Remark

DB_xref: taxon:4565

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: SK primer.

Note: Vector: Lambda Uni-ZAP XR, excised phagemid pBluescript SK(-); Site_1: EcoRI; Site_2: XhoI; Hydroponic plants grown to full tillering stage were treated with 150 mM NaCl for either 12 hours or 7 days. Root tissues of the plants subjected to both types of treatment were collected separately at University of California, Davis (E. Akhunov and K. Deal in J. Dvorak's Lab). Total RNA was prepared separately from the two samples (12h and 7day treatments), and equal amount of RNA was then pooled. PolyA RNA was purified from the pooled RNA, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript SK(-) phagemids in J. Dvorak's lab (E. Akhunov, J. Dvorak) at the University of California, Davis. Colony plating, plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

DNA

tcggcacgaggtaaaggatgacatattctggaatttctttaagacctgtg
tgtttggatctgagagttctgagaattatcctgagttagaatgcattggt
agaagaatacttcctaaactgaagggctctcctttggccgccaaaactct
gggacgcatgttaagcatggaccttcaagaatcacattggaaatttatac
ttgagagtgaattgtggcagttaaaagaaaaggagactgagattttgccc
gcccttcggttgagctatatgtacttaccattttatttgaagcaatgctt
cgcattctgtgctgtgtaccctaaagattacatattccagaagcaacact
tagctgaaatttgggtagcagaaggctttgtagagcctcaaggtggtgtt
ccaattcaagatattggttgtcagtattttgaagaccttgtagcacggtc
cttctttcgaaaagttagtggtggatacgtaatccatgacttgctgcatg
acatggcacaacaagtttcagagcacgactgcttcatcttaagaaacgag
agtgactttgataaagttccccagaatgtccgtcatctatacgtactccc
tagcagtgactttgacgattccagcttgttgagactatgcaagtacacaa
agctgcgtacactaattttgca


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