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GrainGenes Sequence Report: Ta.5610.1.S1_at

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Sequence	BQ752931
Contig	Ta.5610.1.S1_at NSFT03P2_Contig14673
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC283097
NCBI UniGene	Ta.5610
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Hemoglobin Hb1'
Keyword	EST
Species	Triticum aestivum
Cultivar	Chinese Spring
Clone Library	Wheat salt-stressed root cDNA library
Tissue	Roots
Developmental Stage	Full tillering
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE4120_G11_N22ZS Wheat salt-stressed root cDNA library Triticum aestivum cDNA clone WHE4120_G11_N22, mRNA sequence.
Strain	lab_host E. coli SOLR
Clone	WHE4120_G11_N22
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid pBluescript SK(-); Site_1: EcoRI; Site_2: XhoI; Hydroponic plants grown to full tillering stage were treated with 150 mM NaCl for either 12 hours or 7 days. Root tissues of the plants subjected to both types of treatment were collected separately at University of California, Davis (E. Akhunov and K. Deal in J. Dvorak's Lab). Total RNA was prepared separately from the two samples (12h and 7day treatments), and equal amount of RNA was then pooled. PolyA RNA was purified from the pooled RNA, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript SK(-) phagemids in J. Dvorak's lab (E. Akhunov, J. Dvorak) at the University of California, Davis. Colony plating, plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA	cggcacgaggaccggatcttgaccgaccgaaccattcagtgctcgcgctc actcacgcatcatagccaagttaagcgggaaggaaggaaggaaggaagcc atgtctgccgcggagggagccgtcgtgttcagcgaggagaaggaggcgct ggtgctcaagtcatgggccatcatgaagaaggattccgccaaccttgggc tccgcttcttcctcaagatcttcgagatcgcgccgtcggcgaggcagatg ttcccgttcctgcgcgactccgacgtgccgctggagaccaaccccaagct caagacccacgccgtgtccgtcttcgtcatgacgtgcgaggctgctgcgc agctgcggaaagccgggaagatcaccgtcagggagaccaccctgaagagg ctgggcggcacgcacttgaaatacggcgtggcagatggccactttgaggt gacgcggttcgctctgctcgagacgatcaaggaggcgcttccggcggaca tgtgggggccggagatgaggaacgcgtggggcgaggcctacgaccaactg gtcgcggccatcaagcaagagatgaagccctctgagtagctcatccattg tactcatatcatatgccacgcaacttccgtccatatccgtccaactttcg ttgcttgaccggttcactcatgtcaccatattgtgtttgtattgtgtgtt tacgtgtactaacgcatattgtaaaatgggcattcaataaaggaacaaat tgtgcatgaaaaaaaaaaaaaa

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