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GrainGenes Sequence Report: BQ665659

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Sequence
BQ665659
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC155683
NCBI UniGene
Hv.7970
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'Transcribed locus, strongly similar to NP_001063775.1 Os09g0534800 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HX
Tissue
apex
Developmental Stage
apex (3-5 mm in size)
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
HX04J07u HX Hordeum vulgare subsp. vulgare cDNA clone HX04J07 3-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold
vulgare
Clone
HX04J07
Probe
baak1g13
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 603 Std Error: 0.00; Plate: 4 row: J column: 7; Seq primer: M13uni.
Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.
DNA
gaaaggaaagccatatcttataacttagcttcgttccgaatccgacaggt
ccatgcacataaaattaggcgggggatatacatggcccggtcaagtgctc
acaacaactctccgacaaatctaatttcaaaagttatgtgagaagaaaag
gaaggaagaaagagcgagagagcgagagaaacgaagaaatggctgcgact
cggctcctatggtgtgcacaggttcttcaggtcttcctccttggcgtacg
agttcgggatgagcctcgcagcgtaggggtacaggtccttgtacgaattc
ctgatggtgccttctgccactccggtggccaaggagatatctttaagcgg
ctttttgtcctctgaaagctgggttatcatataaatgacagcagccgcaa
ttgaaatagggctcctccttatatcaagctcctctgagcgctgaactgcc
tcctgagctgctttaacagcagtattgttcataccaagagttgagcagaa
acgcctcaggaaatctccagcatggatggttcccatctccatagattggc
ccatttcaacttccagctgtttcacgatgaactcttttgctcgaccaatc
tct

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