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GrainGenes Sequence Report: BQ665513

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Sequence
BQ665513
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC184013
NCBI UniGene
Hv.22806
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'Transcribed locus, strongly similar to NP_724345.1 histone H3 [Drosophila melanogaster]'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HX
Tissue
apex
Developmental Stage
apex (3-5 mm in size)
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
HX03P07u HX Hordeum vulgare subsp. vulgare cDNA clone HX03P07 3-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold
vulgare
Clone
HX03P07
Probe
bast03G0313
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 557 Std Error: 0.00; Plate: 3 row: P column: 7; Seq primer: M13uni.
Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.
DNA
acctggtaggaaaaaggccgatcaatcttacggcaacacaaacacaataa
caaagcagaacagaatcaccaaatcgaaggagaatgttaaatcaattgct
tttcaattttcagtcacctaattaccagggaacaagataaacaaaggaca
gcatgggacgaatttgcatctgaagataactgaacttttcattgccattt
catttcagcatcacgacatcaggaaagatcaccagacagcaggcatcata
tcagcgctacagagacacccagcacgtttccttactacaaccagatcgca
agacacgaactgctacagcggcgaccgaaaacctaggcgcgctcccctcg
gatgcggcgggcgagctggatgtccttgggcatgatggtgacgcgcttgg
cgtggatggcgcagaggttggtgtcctcgaagaggccgacgaggtaggcc
tcggcggcctcctgcagggcggagacagcggaggactggaacctcaggtc
ggtcttgaagtcctgggcgatctccctcaccaggcgctggaaggggagct
tgcgaat

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