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GrainGenes Sequence Report: BQ665438

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Sequence
BQ665438
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC168556
NCBI UniGene
Hv.1014
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'CDNA clone: FLbaf64h18, mRNA sequence'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HX
Tissue
apex
Developmental Stage
apex (3-5 mm in size)
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
HX03J21u HX Hordeum vulgare subsp. vulgare cDNA clone HX03J21 3-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold
vulgare
Clone
HX03J21
Probe
BaH15P22
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 485 Std Error: 0.00; Plate: 3 row: J column: 21; Seq primer: M13uni.
Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.
DNA
gaaataagtaaactgcatacccttctgctgttgcatagcaacacaacatt
attatgtaactggtacaacaaaaacaatgcttgtaagcaaagaagcagcg
gtatggtacggtacagacaccaccgcgacctgcgcagatatctatctcgc
ctgcgagaccgcgtgcgataactgagatggaactcaacacaaagaccggc
tacaaataacttaggacacctcatgcccacatcaactctgatctgatgca
cctccttccttgctggggtgcccaaaccctatcttccatggatatcacat
ttggggacttggtctgggggcaccgacctttcggagtctgcaccagggca
gcggtaacatcggcttgcctctgagttattggtctctgaatagggtggag
agcagcaggtcttgcacgaatgccgctctcacgctggcttgctccctttc
ttcccttgtgagggacaagtcaatacttgaccgcc

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