GrainGenes Sequence Report: BQ665269
Sequence
BQ665269
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC179992
NCBI UniGene
Hv.17741
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare UniGene title 'Transcribed locus, moderately similar to NP_001044962.1 Os01g0875300 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HX
Tissue
apex
Developmental Stage
apex (3-5 mm in size)
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
HX02N04u HX Hordeum vulgare subsp. vulgare cDNA clone HX02N04 3-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold vulgare
Clone
HX02N04
Probe
GBM1405
Remark
DB_xref: taxon:112509 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 566 Std Error: 0.00; Plate: 2 row: N column: 4; Seq primer: M13uni. Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.
DNA
gctccaatactagtatgtcttttgtgcatcacaacacgacggtactacat
caggtacatattacaatcttgcagcagaaagagaaagtctagcagcagaa
aagataaaaaagagaaggggcacaaattacacgcactgaaaagacggcaa
ggctaaggctataggagggtcaaatcctaggactctgtggggctttacat
tttacaacacgatgggggcagcaggagcagcacgatccaccgcagcgaca
cacgaacgacatggccgcacgcacgcacgcacgcacggacatcttttttt
attttttatactcgatggacgggttaaaaaacacatagaatgacagatac
gctggtgtaaaatactcagatcacgcaggagctagacagacaaactcagc
agcgagcttttatagaaacacggtcgtggacatggcggacgggacgacgg
acggtcaagcgaggagccagaagttcttctgccaccgggtgctgacgagg
tatccgccgacgcccttgctctgcttcctcaccgccagcgtcaggcactc
ggcctggttgatgcac

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BQ665269
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