GrainGenes Sequence Report: BQ665060
Sequence
BQ665060
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC178509
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HX
Tissue
apex
Developmental Stage
apex (3-5 mm in size)
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
HX01P07w HX Hordeum vulgare subsp. vulgare cDNA clone HX01P07 3-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold vulgare
Clone
HX01P07
Probe
GBM1212
Remark
DB_xref: taxon:112509 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 144 Std Error: 0.00; Plate: 1 row: P column: 7; Seq primer: T7. Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.
DNA
gggactctctgctttcctttgttgttgttgcaagaagcaaggatggatta
ccagctacggactgactctagagagactgcgacgacgaggaggaggagga
ggacgagtgacgacgacgagagagtaagcgcgcgagtgggaggg
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BQ665060
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||