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GrainGenes Sequence Report: BQ665040

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Sequence
BQ665040
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC156957
NCBI UniGene
Hv.11222
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'Transcribed locus, moderately similar to NP_001058017.1 Os06g0604400 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HX
Tissue
apex
Developmental Stage
apex (3-5 mm in size)
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
HX01O06w HX Hordeum vulgare subsp. vulgare cDNA clone HX01O06 3-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold
vulgare
Clone
HX01O06
Probe
BaAL16O08
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 673 Std Error: 0.00; Plate: 1 row: O column: 6; Seq primer: T7.
Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.
DNA
gagatgaagcattgccaaatcttcaagcttcagtactctcaagtatgcat
cttgcaagacagaccaaggctagccagcagaatgttcaggctttaggtaa
gacgatcagctatagactctgactagaaccatcatatagaggtagcaaca
atagaataagcttacagtgcgggaagcgcatcatatctagctagaagcat
catctagcattgccgaggttgcaactatgcaaatgcatcctatgtggtga
gaatagggggcaggtgtttggatttgattccaagcaccggagcctgcgtg
tcggggaagcacctcgcaccgggcagctccgtcaccgtgccgtcctttgt
cacggcaaccgggtaggtgagaaggtgcccagggaggtcgtcgttcagtt
catcactagcatagaggtcccagtacttgtcagccatcttgttcaccctc
tggacacactccaggcttcccgggctgacgaaatcgttgtgcagcattcc
aaggtgctcataccacagcgacatccggaagccatggacctgccctctgg
caacctggcctttggcgttcaggtggtgtggctggaatgcgcccatggca
atctcggagtccctccccccgtccattgaccgctggttgatgttggcaga
tccgacgatgatgtactcgtcat

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