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GrainGenes Sequence Report: BQ663667

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Sequence
BQ663667
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
NCBI UniGene
Hv.4681
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'Transcribed locus, moderately similar to NP_001131275.1 hypothetical protein LOC100192588 [Zea mays]'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HU
Tissue
germinating seeds
Developmental Stage
germinating seeds, 16-48 h
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
HU03I13u HU Hordeum vulgare subsp. vulgare cDNA clone HU03I13 3-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold
vulgare
Clone
HU03I13
Probe
GBM1404
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 486 Std Error: 0.00; Plate: 3 row: I column: 13; Seq primer: M13uni.
Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); germinating seeds, 16-48h. Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable. Average insert size is 1.2 kb
DNA
ggtaccaaatgactctacttgtagattagatggccttctcagtttgggtg
catttcctaaaaacaatttgctggaatagctacaataacttccagttcct
gatttaaacagagcgataaaatacaacatttaggtgaatgcaaccaccat
aaagcagattatacgctaagtcgatcagtctcccggcaagccagcaaaca
aacaaaaaatccagacatcacaaaatctatgtatgtatgtatgtatgggg
ctacaagctaaagaaagctccactcagtgaccaaaagaatccatctagca
aggaaccatgatcgacacaacaaaaataataatagctcatccgtccatca
actatccttggcagctcaaaagcaaagctccgacgacgcgctccacattt
gcctccctcctccgttcagtctgtcgagttctcatggagcaacacgtact
cccggctactattgccaaacttaatgttgtcccttt

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