GrainGenes Sequence Report: BQ663016

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Sequence

BQ663016

External Databases

TIGR Gene Index

NCBI UniGene

DB Remark

Locus Source: Hordeum vulgare subsp. vulgare

UniGene title 'CDNA clone: FLbaf46f15, mRNA sequence'

Keyword

EST

Species

Hordeum vulgare subsp. vulgare

Cultivar

barke

Clone Library

Tissue

endosperm early

Developmental Stage

0-16 hours after imbibition

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

HT01E15w HT Hordeum vulgare subsp. vulgare cDNA clone HT01E15 3-PRIME, mRNA sequence.

Strain

lab_host XL10-Gold

vulgare

Clone

HT01E15

Probe

baak38d20

Remark

DB_xref: taxon:112509

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 674 Std Error: 0.00; Plate: 1 row: E column: 15; Seq primer: T7.

Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.

DNA

gtaaagacagtggccttgagaaaagccgcctgtcggctatattttaagtt
aacggttgggaccgacatacacggtccaaatggctttgttactttatact
tacacaccactcaccgagactccctggccaacgccaaccctaacagaatc
cggaagcacaaactgggacacgtctcagaacgttacactcagggcaatga
cgtaaagtacgtaaataacatggctctgcactggcactctggcagcacac
cattactcctttggtgattcatgcaagccagcaaatccgatgtcgccgct
gcctccagccggtcgctcatcggcgggacatcttcaaggttgcggttcgt
gctggggcgtggcaggagaatctggtgctatggtgaaatgcactcctctc
ccttgcccagactggaggatgaatgccacctcagttgctgccaacgcagc
tgcctcctgccgatgtctgcgacgctgcaatatactaattgcccaagcca
ttatatagaatgggagaagaaaaccagcagcacgcagtaagaaaagtgaa
aacattgctgaagtgtcatcatcatcttcatcagttagtgtcaatgcatg
ccttagtaataggagagccattaaaacaagagcagcagaacggcaaaagg
cagcagtgctggcattcgtggctg


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