GrainGenes Sequence Report: BQ662872
Sequence
BQ662872
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC192502
NCBI UniGene
Hv.26161
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare UniGene title 'CDNA clone: FLbaf166m05, mRNA sequence'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HS
Tissue
embryo + scutellum
Developmental Stage
0-16 hours after imbibition
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
HS04I19u HS Hordeum vulgare subsp. vulgare cDNA clone HS04I19 3-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold vulgare
Clone
HS04I19
Probe
UMB006 GBM1215
Remark
DB_xref: taxon:112509 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 450 Std Error: 0.00; Plate: 4 row: I column: 19; Seq primer: M13uni. Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.
DNA
aatatagcatcagccgatgaccagaaaacgcctgtcaaaatcggctggcg
ggctgataacacaagcaggcgtatccggaacacgtcgaccgcaaatggca
gctccctccctgcacggtgcagctcaaaaccaccggagccagacgcggcg
catctccaaaagcatgacacacacacacacacacacacacgcccacggga
acaaaagaagaggttcgttttctcgtgtgtgcagaatccggaaaagagca
aaggcaaaggttctcgaacgggcgcccgttcgtcggagtcgctttcgaga
ttcggacgacgagcaacggggcgcgccgtgacagcaacggagcatgctgg
taggcaggcatcggcgcgagagacggaatcggaatcggggcggtagtgca
ggccgccttcggtcaccgctttgcccgttgctaccaccgttgctgtcagt
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BQ662872
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||