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GrainGenes Sequence Report: BQ662756

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Sequence
BQ662756
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
NCBI UniGene
Hv.1933
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'Transcribed locus, strongly similar to NP_001056762.1 Os06g0141700 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HS
Tissue
embryo + scutellum
Developmental Stage
0-16 hours after imbibition
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
HS04A16u HS Hordeum vulgare subsp. vulgare cDNA clone HS04A16 3-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold
vulgare
Clone
HS04A16
Probe
bast42C0406
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 518 Std Error: 0.00; Plate: 4 row: A column: 16; Seq primer: M13uni.
Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.
DNA
agtttcaatttcgacgacagatattcaattattcattccgcagcagtttc
ttgggacactgacgacgatgcatacattagcaggaacactagctcatggg
ctgaagaacgccggtggcggtggacgtggacgagcggcggcgccatggaa
ggaggtgtccttgaggtggtcgggggcgctctcgtacgagtgcctcctcg
ccagcgagaggatggtcttgtcggcggcgacgaagtaggccatcccggcg
acggtgcagacgatgagcgcctggccggtggggttcaggttcgccttagc
ccacggcagcatcctcacgctcgtcagcgttgcctcgagttttttttttt
tttttttagacaaacgagatatgttgacataacagtagggatgatacatt
gtttacatatgacaaaatttgcgcattttgacacggtatatgacaaaact
ggcactgtgaagggtccactaacatacttcagtgaagaaaaagaatacaa
cgatgctactcaagaaat

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