GrainGenes Sequence Report: BQ661885
Sequence
BQ661885
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC176328
NCBI UniGene
Hv.12056
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare UniGene title 'Transcribed locus, moderately similar to NP_001050822.1 Os03g0659900 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HP
Tissue
epidermis
Developmental Stage
seedlings grown in greenhouse for 6 days
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
HP01K21w HP Hordeum vulgare subsp. vulgare cDNA clone HP01K21 3-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold vulgare
Clone
HP01K21
Probe
GBM1143
Remark
DB_xref: taxon:112509 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 463 Std Error: 0.00; Plate: 1 row: K column: 21; Seq primer: T7. Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 850 bp
DNA
gtgcaacaagtcattccattcgtctgcagcattcggcaccactactacat
gttacacctcgcacccacacactggagtacaaaaagaatcgcacaacact
cactatcaacggcgtttttttgtacaaggctccaacggcactagcaattt
acatacactagacccaacaaccaaaaagagagagagagaggcatatggga
gataacacggcagcatttccggcccgcaagccgctacgcaagcatattcg
tcattatcctttgatgaaggggggcgggcgggctagcatcatcagcttat
aacgacaccggaccatcgctttgtccaatccgcccgccatgctcggcatt
tcatcttttatatggtccgccttcatattttccgtctccacggtttctat
gtcacctgctcgttctcgtttctgggcgggtcggcgtagtcctgaccttg
gtggtcgcgaacg

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BQ661885
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