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GrainGenes Sequence Report: BQ661796

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Sequence
BQ661796
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC158975
NCBI UniGene
Hv.24813
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'CDNA clone: FLbaf148i18, mRNA sequence'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HP
Tissue
epidermis
Developmental Stage
seedlings grown in greenhouse for 6 days
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
HP01F10w HP Hordeum vulgare subsp. vulgare cDNA clone HP01F10 3-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold
vulgare
Clone
HP01F10
Probe
basd15e10
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 666 Std Error: 0.00; Plate: 1 row: F column: 10; Seq primer: T7.
Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 850 bp
DNA
catggattgtgatcagaacgatcaaaccgatgtaagttacaacattcaag
cccgacaaatcttgcattgcttttgcagaaaactctcgtaatttacatca
tggtagtacaactcagattgcacaacaacatgtttcatggcatgcacaat
cttcaaggcacatcttacttgccggggacaaagttggtggcaaacgccca
ggcattgttgttgatggggtcagcgatgtggtcagcgaggttctcaagtg
ggcctttgcccgtgacgatagcttggacaaagaagccgaacattgagaac
atggcaaggcgaccgttcttgacctcctttaccttgagctcagcgaatgc
ctcggggtcgtcggctaggccaagagggtcgaagctccctcctgggtata
gtgggtcgacgatctcgccgagtgggccgccagcaacacggtacccctcg
acggcacccatgagcacaacctggcatgcccagatagctaggatgctttg
cgcgtgcacaaggctggggttgcctaggtagtcgagaccaccctcactaa
agatctgggagccagccttgaaccaaacggcctctccaaacttgacaccg
ttgcgggcgagcaactcagggaagacacaaccaagtgccccaagcatagc
ccaacggcaatggatc

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