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GrainGenes Sequence Report: BQ661747

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Sequence
BQ661747
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC170765
NCBI UniGene
Hv.23380
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'Hypothetical protein LOC548250'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HP
Tissue
epidermis
Developmental Stage
seedlings grown in greenhouse for 6 days
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
HP01B24w HP Hordeum vulgare subsp. vulgare cDNA clone HP01B24 3-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold
vulgare
Clone
HP01B24
Probe
GBM1139
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 480 Std Error: 0.00; Plate: 1 row: B column: 24; Seq primer: T7.
Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 850 bp
DNA
agataaacatggggttcctccattaattttactgcatataaatcgataaa
aaggagtacaagcccctgcttgatcgatgagaaatgcatctgatccgagc
acacgaacactacagcagggcatccaagcccgtccacccacccacgcacg
cacacacaatgggaaacagcccaacatccaggccgcatttccccccgtag
taataggatcgacgatgatgatgatgatgatgcgtcgttaacccgcgcgg
ggctcggcgctcgagcgccccctgccttccctcattagtcgttatacgta
gcatagcggttttccttccatggctggcgggaccaggagggtcgagctga
tcgggggcttcaagcccgggcgtaggtctgcccgaggtgcgcggcgcgga
ggtcggcggggtcggcgccggaggagcggttggcggggcggtagtggccg
gtgacggggtcgggcacccacgaggagcct

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