GrainGenes Sequence Report: BQ661625

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Sequence

BQ661625

External Databases

TIGR Gene Index

NCBI UniGene

DB Remark

Locus Source: Hordeum vulgare subsp. vulgare

UniGene title 'Transcribed locus'

Keyword

EST

Species

Hordeum vulgare subsp. vulgare

Cultivar

barke

Clone Library

Tissue

male inflorescences

Developmental Stage

male inflorescences (approx. 2 mm in size), green anther stage

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

HM04G20u HM Hordeum vulgare subsp. vulgare cDNA clone HM04G20 3-PRIME, mRNA sequence.

Strain

lab_host XL10-Gold

vulgare

Clone

HM04G20

Probe

GBM1389

Remark

DB_xref: taxon:112509

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 354 Std Error: 0.00; Plate: 4 row: G column: 20; Seq primer: M13uni.

Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.

DNA

acgaagcaaactgagcccaggcttccatcatcgttctttttctcatctta
cgacaaaagaaaatgacaggtggttttatgtacagaggaaatgcaaaggg
cggatcgacaacaaagggtaaatcatctactgctactactcctaattggc
taataatccattcgcacaccaagagtcaacagcctgcctgcctgcctgcc
taccaaagcaaccagacaccaacggcacagcaagaaacccaagaaagaag
caacaggatcaagcaaaagcaggggaagggggcggggacgggctgggcta
atctagctctggtaactaattttgcacggtggcgggcggcggttcctcgt
gccg


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