Query (optional)   in Class  

GrainGenes Sequence Report: BQ660920

[Submit comment/correction]

Sequence
BQ660920
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC164163
NCBI UniGene
Hv.9774
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'Transcribed locus, moderately similar to NP_001056580.1 Os06g0110100 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HM
Tissue
male inflorescences
Developmental Stage
male inflorescences (approx. 2 mm in size), green anther stage
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
HM01B17w HM Hordeum vulgare subsp. vulgare cDNA clone HM01B17 3-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold
vulgare
Clone
HM01B17
Probe
GBM1126
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 577 Std Error: 0.00; Plate: 1 row: B column: 17; Seq primer: T7.
Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.
DNA
cgctggaccagatttcccatatacaccatcaaatctgagatgccaaagca
caaagatacagacttgcaacaacagccaaccaaccggttacaacttacaa
cagcagatgattccccagatgacagtaagatggaccaacactggcagaat
acgattcaaacatgccggaacggatcgaactatatggccgggcgctcttt
atttatctcacaggcgtctccggatcctccaggcagggagggcaatatgc
ctcctatatatatatatggtatctccacaatgcggttcgcttgaactacc
acctgactggcctcctcctcggcgatcgatgcctcaagacatctacaccg
tgggcatcggaactcaaccgtctttacccgatgactggtacacagcgtcc
atgatgccatggatcgtcgacaggctcaggccagggaacctgtgaggagc
gtcggggcggatctcaatctttacgaacggccatttcattatggaagcag
cgctgcgctcctctgggccgtctccaaccgcgcagaagcggacgttcggg
ccgccgtggcgttctttgatccacttg

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.