GrainGenes Sequence Report: BQ660219

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Sequence

BQ660219

External Databases

TIGR Gene Index

NCBI UniGene

DB Remark

Locus Source: Hordeum vulgare subsp. vulgare

UniGene title 'Transcribed locus, weakly similar to XP_002283829.1 PREDICTED: hypothetical protein [Vitis vinifera]'

Keyword

EST

Species

Hordeum vulgare subsp. vulgare

Cultivar

barke

Clone Library

Tissue

female inflorescences

Developmental Stage

female inflorescences (approx. 3 mm in size)

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

HI01I18w HI Hordeum vulgare subsp. vulgare cDNA clone HI01I18 3-PRIME, mRNA sequence.

Strain

lab_host XL10-Gold

vulgare

Clone

HI01I18

Probe

GBM1218

Remark

DB_xref: taxon:112509

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 525 Std Error: 0.00; Plate: 1 row: I column: 18; Seq primer: T7.

Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.

DNA

aaagaggaataaccgtttccttgatgcatgcatcaaccaggtgattatcg
tctcatacagaacgtgactggcactcacaacacaggagctacgagagaga
gagagagagagagagttacagctagcaatggcgaccagcaagcatcgatc
tccgaccatgtcggtgcagttggccgaccaacctagtcaaggatggaagc
gctgcatgcaggcgtggcaggtgatctcgagcgcggcggcggaggcggcg
gagccgagccgcctggcggcgatctgcttcatccgctccacccgctccac
ctcctcgcgggcgcgctcccagatcatcctcgcgcgcgcgaactccctct
cggcgagctccagctcccgcttcgccagctcccggacgcgctccgcgtag
gcgcgctccgccgacgccatccgcgcctgctccgccgtctgctgcttcac
ggcgcggacgtccaccgtcacctgctgctgctgctgctgcagctgcctcg
cagcgctggcggccacggtcgccgc


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