GrainGenes Sequence Report: BQ660019

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Sequence

BQ660019

External Databases

TIGR Gene Index

NCBI UniGene

DB Remark

Locus Source: Hordeum vulgare subsp. vulgare

UniGene title 'Fungal pathogen induced mRNA for pathogen-related protein'

Keyword

EST

Species

Hordeum vulgare subsp. vulgare

Cultivar

barke

Clone Library

Tissue

green leaves

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

HG01K10u HG Hordeum vulgare subsp. vulgare cDNA clone HG01K10 3-PRIME, mRNA sequence.

Strain

lab_host XL10-Gold

vulgare

Clone

HG01K10

Probe

GBS554

Remark

DB_xref: taxon:112509

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 445 Std Error: 0.00; Plate: 1 row: K column: 10; Seq primer: M13uni.

Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 800 bp

DNA

ggataaaaaatctttttattgatttaaatatttcttgccattacaaggaa
ttttgccaaatccgaaatcctggagacgatgatacaagtcacatgtttcg
ccaattctgttgcgcttcagtctgctcctctcatcacagggcaacctgaa
gccgatgcagaagcagccggcgcagtcaagaagctcgcgaggaagttgcc
acgctcgtagaagaactctgccttctccaccttgatgtcctcatcaacat
ggaagacgcaaacgccgaagaactcgacgcgcccaccgtgcggagggtgg
cccttgaagggaccctccatgtaaccccagtgccggaacttgaaggcgat
cctgggagctggcgggccgctgtagacgtccagcacctcgatggcgaagc
cccgggggaacgccgtgaggaacgtggacgtcgccgactcgaccg


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