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GrainGenes Sequence Report: BQ659575

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Sequence
BQ659575
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC154305
NCBI UniGene
Hv.25587
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'CDNA clone: FLbaf23a10, mRNA sequence'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HE
Tissue
etiolated leaves
Developmental Stage
grown for 6 days in growth chamber (harvested at daylight conditions)
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
HE01O18u HE Hordeum vulgare subsp. vulgare cDNA clone HE01O18 3-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold
vulgare
Clone
HE01O18
Probe
baet43H1016
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 496 Std Error: 0.00; Plate: 1 row: O column: 18; Seq primer: M13uni.
Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 900 bp
DNA
ccttttttttggacaaaccaaacaatttcttcgcacataatctctcataa
tatacatgttggtgttacaactcatgtgacactcacatacaactcgcatt
catcactcatgtcttacttcccgggcacaaagttggtggcgaatgcccac
gcattgttgttgacagggtcgacaatgtggtctgcaaggttctccagtgg
gcccttgccagtgacaatggcctgcacaaaaaagccaaacatggagaaca
tggcaaggcgtccattcttgatctccttaaccttgagctccgcaaatgcc
tcggggtcgtcggccaggccaagcgggtcaaagctgccaccaggatagag
tgggtcgacgatctcgccgagcggaccaccagcaatgcggtacccctcaa
cggcacccatgagcacaacctggcaagcccagatggcgaggatgctctga
gcatgaacgaggttggggttgccaaggtagtcgaggccaccctcgc

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