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GrainGenes Sequence Report: BQ658775

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Sequence
BQ658775
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC157425
NCBI UniGene
Hv.8999
DB Remark
Locus Source: Hordeum vulgare
UniGene title 'CDNA clone: FLbaf168e11, mRNA sequence'
Keyword
EST
Species
Hordeum vulgare
Cultivar
Golden Promise
Clone Library
HD
Tissue
callus
Developmental Stage
callus (5-10 mm in diameter)
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
HD03I16u HD Hordeum vulgare cDNA clone HD03I16 3-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold
Clone
HD03I16
Probe
baak42a24
Remark
DB_xref: taxon:4513
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 506 Std Error: 0.00; Plate: 3 row: I column: 16; Seq primer: M13uni.
Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 1 kb
DNA
gaagcaaatgtttgtttcgagctcacaagcaaagatacagaatttggcta
ttcaaagtgaacaaacaatttatctcagcttagccggccaacagattttg
cattgtacacgctaatcagagacatttttccctagggaaggcagagagcg
caggggagaccaaacccattatgtatgtctgctgcgcaacgccagcagcg
tatagcaatgttagtacaacacaaggatagaatccccatggtttttggct
tctgacggtcacctccacctggcgcctcccaatctgatcacctcttcagt
tccggaatccctgcggggggctctatcatcgccagcatcgtcctttgcgt
ttgtagcatcaccgtacagatccttcagcttcgccaggttggatgactcc
ggcgctttaccaaacgggctggaacgttggctgtcgccattggtcctgcc
acggcttggactcctgctcctgctcctgctcctgcttcggcggccactcg
aacgcc

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