GrainGenes Sequence Report: BQ658384
Sequence
BQ658384
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC186774
NCBI UniGene
Hv.20767
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare UniGene title 'CDNA clone: FLbaf66h13, mRNA sequence'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
BC
Tissue
developing caryopsis
Developmental Stage
8-15 DAP (days after pollination)
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
HB09F23w BC Hordeum vulgare subsp. vulgare cDNA clone HB09F23 3-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold vulgare
Clone
HB09F23
Probe
GBS577
Remark
DB_xref: taxon:112509 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 679 Std Error: 0.00; Plate: 9 row: F column: 23; Seq primer: T7. Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); developing caryopsis , 8-15 DAP(days after pollination) Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable. Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); developing caryopsis, 8-15 DAP(days after pollination) Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.
DNA
ctgggtgtatgttaatattgctaccatgatcaacataggcatattgaaat
gtttgctatagacacctcggtggcgcacatttagaaattttatccataca
ttattccaagaccacttttatttgtcacatgcagtgatatatgacagttt
attagctagtcacaactggaagctacatcaccagtaatagccagtagtgc
tggggatgttgcaggcagggccctggttgcacctggggggcaggttcttg
gctgcttgtatcactttgactgtccgatcacgctgaaatccgaagaaacc
accgagatcacgttggattgatccctggatgatgttgcagcggcattgcg
gtggcatttgactcaactgactgcaacaatcgtgaaggagctcttcacaa
cctcccttccaccatgtccaccaacgccaggtgaccggaaaatccttcat
cgttgtgcatcgatctagcaggtaattcctgcaggaatctagctttgtcc
ctagggggcattgttgagcaccacccccaccagcaccaccctcgtaactg
ccaacaacttctccgtactgcgcgaaggcggcgctcgctaccaaagcaag
cagacccacgaggaagaaggccttcatgttgtgacaactgtatcaaacgc
ttctgcaactacaaataccctatgttcat
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BQ658384
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||