GrainGenes Sequence Report: BQ658357
Sequence
BQ658357
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC156877
NCBI UniGene
Hv.15826
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare UniGene title 'Transcribed locus, moderately similar to NP_001056068.1 Os05g0519900 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
BC
Tissue
developing caryopsis
Developmental Stage
8-15 DAP (days after pollination)
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
HB09H09w BC Hordeum vulgare subsp. vulgare cDNA clone HB09H09 3-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold vulgare
Clone
HB09H09
Probe
bastl41G0513
Remark
DB_xref: taxon:112509 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 671 Std Error: 0.00; Plate: 9 row: H column: 9; Seq primer: T7. Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); developing caryopsis , 8-15 DAP(days after pollination) Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable. Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); developing caryopsis, 8-15 DAP(days after pollination) Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.
DNA
ctctacacagtgccaatcataccacttcaacattccaaggtatcataggc
acttgctcagggaggagtggtgatccagtaaatagcaagttgttccactt
gtcagtaattcactggttgacataattcagaatttctactgttcatgcgg
ttctcgctcaattgctacatgggaaatggaatctcatgcgtccgggatta
caccttcctatcctacatttcaaatggaatctccttgcactacagaacca
tacgtccatactggaggttccagaaagaaaaaagtgatgcgcagaaaagc
atacaaaaatgtggatatctttaaccaagttcttctcgtcagtgtgttct
tctgccgagttcgggcactttggccacggcaaatagtgtgtttgtgttct
tcaagggtaaatagtcggcttgtgttctctattcgtctattgccatgggt
tcttctttccagcccctttgcgcattaaacttgttttcgaactagatttc
gatttcaatttcaactttcctcgaaccctgttcttttgcggtttcagttt
gtaaatcctaaccatccagtgctgggttgtaaaaacctcctcaaaatggg
tcagtttaaaatgcttccttcctatttcatatctcctcgctctgtcaaat
cccttaccatcagtctcagcg
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BQ658357
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||