GrainGenes Sequence Report: BQ657691

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Sequence

BQ657691

External Databases

TIGR Gene Index

NCBI UniGene

DB Remark

Locus Source: Hordeum vulgare subsp. vulgare

UniGene title 'CDNA clone: FLbaf162c13, mRNA sequence'

Keyword

EST

Species

Hordeum vulgare subsp. vulgare

Cultivar

barke

Clone Library

Tissue

embryosac

Developmental Stage

0-7 DAP (days after pollination)

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

HA07F23u HA Hordeum vulgare subsp. vulgare cDNA clone HA07F23 3-PRIME, mRNA sequence.

Strain

lab_host XL10-Gold

vulgare

Clone

HA07F23

Probe

GBS687

Remark

DB_xref: taxon:112509

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 579 Std Error: 0.00; Plate: 7 row: F column: 23; Seq primer: M13uni.

Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); 0-7 DAP(days after pollination) Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable. Average insert size is 1 kb

DNA

actctcaagtctcaatgtcttatattacccacatcaagcgctaacaagtg
gccacaataattactgctacatgacagcaaatagattaaatggctaggtg
gattcttaaaactaatcagtaccctcacccttacaaagtgttggctatca
aacattgttcaaagcaacaaaggcatgcacctgaacacaagactgttgac
ataggagttgggacttgtccatgcaatgccacgaaatcaggagagtaggt
ccatagcaaccactaattttcgcaaaaggtgttgacccatgctgagcatg
tgatgtagcactaaatctcagtgaccagctgaggccaattctgcaaaaca
tggaacataaggcagacccatcatgccttcagtgcagataacatgcactt
tctgattgtccatgtcgtacgacaatgtcatgttcttcttgtcagtaagg
aaaatcatattgcaatctggatgaactgcaaacatcgcataagattctcc
atctttacggcaattccttccaaacagctccaaaacattaatagtgtgct
tcagggcccacttcccagtatcataatcc


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