GrainGenes Sequence Report: BQ620664
Sequence
BQ620664
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC309347
NCBI UniGene
Ta.58525
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Golden 2-like protein'
Keyword
EST
Species
Triticum aestivum
Cultivar
Thatcher Lr1
Clone Library
TaLr1
Tissue
Leaf tissue
Developmental Stage
14 Days old
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
TaLr1135D05R TaLr1 Triticum aestivum cDNA clone TaLr1135D05R, mRNA sequence.
Strain
lab_host E. coli XLOLR
Clone
TaLr1135D05R
Probe
MAG510
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Dr. Sylvie Cloutier; Cereal Research Centre, Agriculture and Agri-food Canada; 195 Dafoe Rd, Winnipeg, MB, Canada R3T 2M9; Tel: (204) 983-2340; Fax: (204) 983-4604; Email: scloutier@em.agr.ca; was cloned directionally, not all sequences generated with reverse; primer where from the 5' end (same with forward primer and 3'end).; Average insert size is >2.2kb; Plate: 135 row: D column: 05; Seq primer: M13 Reverse. Locus Comment: Contact: Dr. Sylvie Cloutier; Cereal Research Centre, Agriculture and Agri-food Canada; 195 Dafoe Rd, Winnipeg, MB, Canada R3T 2M9; Tel: (204) 983-2340; Fax: (204) 983-4604; Email: scloutier@agr.gc.ca; was cloned directionally, not all sequences generated with reverse; primer where from the 5' end (same with forward primer and 3'end).; Average insert size is >2.2kb; Plate: 135 row: D column: 05; Seq primer: M13 Reverse. Note: Vector: Lambda ZapII; mass excised in plasmid vector pBK-CMV (Stratagene).; Site_1: EcoRI; Site_2: XhoI; mRNA obtained from wheat NIL Thatcher Lr1 24 hours after inoculation with leaf rust pathogen Puccinia triticina race BBB carrying the avirulence gene Avr1.
DNA
gaattgggcacgaggaccgccccattgtgccggacgcataatcgccgtcg
tcgacaacgtcgtcgtccacggaggccgagagccgccacaagtcatcaag
caagaactcccacggaaagaagaaagccaaggtggactggacgccggagc
tgcaccggaggttcgtgcaggccgtagagcagctcggcatcgacaaggca
gtgccgtctaggattttggagatcatggggatcaactcactcactcggca
caacatagcaagccatttgcagaagtaccggtctcaccggaagcacatga
ttgcgcgggaggcggaggcggcgagctggacccaacgacgacagatgtac
gccgccggcgggccggccgcggccgtgaagaggcaggactcgaacatgtg
gaccgtgccaaccatcggcttcgcgccggcgcaccctcctcctcctcctc
cccctccttcaccggcagccatgcagcactacgctcggccgttgcacgtc
tggggtcaccctacgatggactcgccccggatgccgatgtggccgaggca
cacaatatcccgcgccccgatgccggcgtgggc

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BQ620664
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