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GrainGenes Sequence Report: TaAffx.111358.1.S1_at

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Sequence	BQ578891
Contig	TaAffx.111358.1.S1_at NSFT03P2_Contig8098
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC304992
NCBI UniGene	Ta.13283
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, moderately similar to NP_001067263.1 Os12g0613300 [Oryza sativa (japonica cultivar-group)]'
Keyword	EST
Species	Triticum aestivum
Cultivar	Chinese Spring
Clone Library	Wheat etiolated seedling root cDNA library
Tissue	Root
Developmental Stage	Five day old etiolated seedling
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE0407_H10_P19ZS Wheat etiolated seedling root cDNA library Triticum aestivum cDNA clone WHE0407_H10_P19, mRNA sequence.
Strain	lab_host E. coli SOLR
Clone	WHE0407_H10_P19
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized , germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Roots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Roots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA	ttcggcacgagcctagacctgccgccgaacgtgccctagccaccgccggc gcctcccgcgcgcggtcgacgtctccgccccacatcgccgcgcctccgcc cccgccccgtccgcagcctttgcttcccagatccgcctccgatttgtttg gcgacaggcgctgttgctttcgttgtaggaggaggaggaggaggaggagg aggaaggggagccctagccttctagggcaaggccgaagcagatgggggcg ccgaagcagaagtggacggcggaggaggaggcggcgctcaaggccgggat aggcaagcacggggccgggaaatggcgcaccatactcaaggaccccgagt tcagcaacatcctgcgctaccgatccaacgttgacctcaaggacaagtgg cggaacatgaatgtcaccgtgaacgcgtcgggcactcgcgacaaagtgag gacgacgacgacgacgacgacgcccacggctaagaagccgaggtctgctc cgaagcaggaaacccactcgacggcgatcaccaccatcacctctgacggt gatgatgacgtcgtcgacgtgaagcctatcataaagcctattgtgacgtt tactacaggttcagggaataaatcgctatccaggctagaaaatatcatac tggaggccgtgaagaccttgaatgagcctacagggtcatacaaaacagcc gttgctaattacatagaggaacaatactggccacctgctgattttgatca tgtactgtctgcaaaactgaatgagttgacatcatct

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