GrainGenes Sequence Report: NSFT03P2_Contig15213
Sequence
BQ483589
Contig
Ta.9570.1.S1_at TaAffx.80260.1.S1_s_at NSFT03P2_Contig15213
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC292849
NCBI UniGene
Ta.9570
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, moderately similar to NP_001058805.1 Os07g0124600 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Clone Library
Wheat unstressed root cDNA library
Tissue
Roots
Developmental Stage
Full tillering
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE3510_D01_G02ZS Wheat unstressed root cDNA library Triticum aestivum cDNA clone WHE3510_D01_G02, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE3510_D01_G02
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid pBluescript SK(-); Site_1: EcoRI; Site_2: XhoI; Plants were grown until full tillering stage and root tissue was collected at Texas Tech Univeristy (Zhang, HT Nguyen Lab ). Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript SK(-) phagemids in the TJ Close lab (Close, Fenton) at the University of California , Riverside. Colony plating, plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid pBluescript SK(-); Site_1: EcoRI; Site_2: XhoI; Plants were grown until full tillering stage and root tissue was collected at Texas Tech Univeristy (Zhang, HT Nguyen Lab). Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript SK(-) phagemids in the TJ Close lab (Close, Fenton) at the University of California, Riverside. Colony plating, plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
gaaccctagctctccacaagcctcttcgtcggcgatggcggcggctgaga
acgccggggacgtggcggcgggggagagccgcaagctgttcgtgggtggc
atcccgtcctcagcgcaggagacggagctgcggggccactttgcccgctt
cggtgcggtgcgctcaatcgtcgtgatgcgggacaaggagtcgggccacg
gccgcgggttcgggttcgtcgagttcgaggacgaagaaacggccgccaag
gcgctcggtgacggggagaggcccaagcacttcatctgcggccgactggt
cgacgtta
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: NSFT03P2_Contig15213
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||