GrainGenes Sequence Report: NSFT03P2_Contig7673
Sequence
BQ483479
Contig
NSFT03P2_Contig7673
External Databases
Data at GenBank Data at EMBL Data at DDBJ
NCBI UniGene
Ta.7578
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, moderately similar to NP_001046995.1 Os02g0525900 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Clone Library
Wheat unstressed root cDNA library
Tissue
Roots
Developmental Stage
Full tillering
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE3509_A12_A23ZS Wheat unstressed root cDNA library Triticum aestivum cDNA clone WHE3509_A12_A23, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE3509_A12_A23
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid pBluescript SK(-); Site_1: EcoRI; Site_2: XhoI; Plants were grown until full tillering stage and root tissue was collected at Texas Tech Univeristy (Zhang, HT Nguyen Lab ). Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript SK(-) phagemids in the TJ Close lab (Close, Fenton) at the University of California , Riverside. Colony plating, plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid pBluescript SK(-); Site_1: EcoRI; Site_2: XhoI; Plants were grown until full tillering stage and root tissue was collected at Texas Tech Univeristy (Zhang, HT Nguyen Lab). Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript SK(-) phagemids in the TJ Close lab (Close, Fenton) at the University of California, Riverside. Colony plating, plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
gttgaaagtgcaaaaaatgatgtcacagtaggcatttgtttgacatatga
aaatcatttggccatgaagaaagaggacacacaatggatagctggaagag
atgtttggtgggaggatgttgtgcccaatttcccaactagatgtgatgtg
gaatgggttgatgcagaggatccattgtttcttctatacactagtggcag
cacaggaaaaccaaagggtgtactgcatacaacggggggctatatggtat
atgccgcaacaacatttaaacatgcatttgattacaagccaacagacata
tactggtgtactgcagactgtggctggattactggacatagttatgtgac
ttatggtcctctcctgaatggagcaacagttctcgtttatgaaggggctc
caaactaccctgatcctggtcggtgttgggacattgttgacaaatacgga
gtgacaatattttatactgcaccaacacttatccgttcgctcatgcgtga
tggttccgtggtatgctgtttttttttcttgtgtgctcatttgaacattt
aggctcgtcgtcagttaagtggtgcactgaggcagcacttcttttcctca
caatttttttttgatctaccgggaagctttttctaccatatagtattctt
ttatattgcaaaataatggcgcaacaacatatgctccagagcgaaatctc
taataatgtctgcacgtttatgaaaatttaaaatttaattag
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: NSFT03P2_Contig7673
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||