GrainGenes Sequence Report: NSFT03P2_Contig13529
Sequence
BQ483417
Contig
Ta.10232.3.S1_a_at Ta.10232.3.S1_at NSFT03P2_Contig13529
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC298752
NCBI UniGene
Ta.10232
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, strongly similar to NP_001058410.1 Os06g0687800 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Clone Library
Wheat unstressed root cDNA library
Tissue
Roots
Developmental Stage
Full tillering
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE3508_D02_H04ZS Wheat unstressed root cDNA library Triticum aestivum cDNA clone WHE3508_D02_H04, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE3508_D02_H04
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid pBluescript SK(-); Site_1: EcoRI; Site_2: XhoI; Plants were grown until full tillering stage and root tissue was collected at Texas Tech Univeristy (Zhang, HT Nguyen Lab ). Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript SK(-) phagemids in the TJ Close lab (Close, Fenton) at the University of California , Riverside. Colony plating, plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid pBluescript SK(-); Site_1: EcoRI; Site_2: XhoI; Plants were grown until full tillering stage and root tissue was collected at Texas Tech Univeristy (Zhang, HT Nguyen Lab). Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript SK(-) phagemids in the TJ Close lab (Close, Fenton) at the University of California, Riverside. Colony plating, plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
cggcacgagggactggttcgccctcgccgactcagatggcgacggccgca
tcaccggccccgacgccatcaggttcttcgccatgtccagcctcccccgc
gccgacctcaagcaggtctgggccatcgccgactccaagcggctggggta
cctcggcttcggcgagttcatcacggcaatgcagctcgtctctctggcgc
aggcggggaacgagatatcccaggacagccttcagcgcgaagatctaatc
agcttcaatcccccagtgatggagggtttggatgctcaacttgccaaatc
caagcatctggcaaagagggtcgatcaggatatggatggatttcctcagg
cacaaggaccttctactaaccattggttcaactctaagtcatccaagaag
atacccctgactgctgttacttctgtcattgatggtttaaaaaggctgta
catcgaaaaattgaagcctttggaagttacatacaaattcaatgattttg
tgtccccgttactgacaaatagtgattttgatgcaaagccaatggttatg
ctcttaggtcaatattctacaggaaaaactactttcatcaagcatctgct
caaaacaagctatccaggtgcccatattggtccggagccgacaaccgaca
g
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: NSFT03P2_Contig13529
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||