Query (optional)   in Class  

GrainGenes Sequence Report: TaAffx.43552.1.S1_at

[Submit comment/correction]

Sequence
BQ482880
Contig
TaAffx.43552.1.S1_at
NSFT03P2_Contig4760
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
DB Remark
Locus Source: Triticum monococcum
Keyword
EST
Species
Triticum monococcum
Cultivar
DV92
Clone Library
Triticum monococcum vegetative apex cDNA library
Tissue
Vegetative shoot apex
Developmental Stage
Three weeks-old plants
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE0573_E10_J19ZE Triticum monococcum vegetative apex cDNA library Triticum monococcum cDNA clone WHE0573_E10_J19, mRNA sequence.
Strain
lab_host E. coli XLOLR
Clone
WHE0573_E10_J19
Remark
DB_xref: taxon:4568
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: T3 primer.
Note: Vector: Lambda pBK-CMV (Lambda Zap Express), excised phagemid; Site_1: EcoRI; Site_2: XhoI; The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised at the University of California, Davis (V. Echenique, B. Stamova , J. Dubcovsky). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
Note: Vector: Lambda pBK-CMV (Lambda Zap Express), excised phagemid; Site_1: EcoRI; Site_2: XhoI; The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised at the University of California, Davis (V. Echenique, B. Stamova, J. Dubcovsky). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
ggcacgagtgattgttattgggcctccaatcaattctactgatgaaaagg
tggttgagattcattcatggaccccggatgggcaaccccctgtgtggaat
ttggttgccacacggccataccggggcggccagattctgtgcgccgtgat
gggttgctgagtcggtcaactgaataaaggttggacgcatgcaagatatg
atgctggctgtgcctcattctcagtctgaatttcctgagagagctccgct
gatgatgatataaaaagagaagacatgaatgagttccagagaaagtgcct
gcagaaacatccgagttttagtcactagctgttatttccaattgcaattt
gtgttccaataatgttttgacactttgtacggtgttctgatgatcttcat
ctgcatcaaccaactttatccgtacagtaaaaaaaaaaaaaaaaaaaa

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.