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GrainGenes Sequence Report: NSFT03P2_Contig12025

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Sequence	BQ482863
Contig	NSFT03P2_Contig12025
External Databases	Data at GenBank Data at EMBL Data at DDBJ
DB Remark	Locus Source: Triticum monococcum
Keyword	EST
Species	Triticum monococcum
Cultivar	DV92
Clone Library	Triticum monococcum vegetative apex cDNA library
Tissue	Vegetative shoot apex
Developmental Stage	Three weeks-old plants
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE0573_D04_H07ZE Triticum monococcum vegetative apex cDNA library Triticum monococcum cDNA clone WHE0573_D04_H07, mRNA sequence.
Strain	lab_host E. coli XLOLR
Clone	WHE0573_D04_H07
Remark	DB_xref: taxon:4568 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: T3 primer. Note: Vector: Lambda pBK-CMV (Lambda Zap Express), excised phagemid; Site_1: EcoRI; Site_2: XhoI; The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised at the University of California, Davis (V. Echenique, B. Stamova , J. Dubcovsky). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda pBK-CMV (Lambda Zap Express), excised phagemid; Site_1: EcoRI; Site_2: XhoI; The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised at the University of California, Davis (V. Echenique, B. Stamova, J. Dubcovsky). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA	ggcacgaggttgggtgaagaacagaagatgtgttgttgtttccttatcca ttaaacctaggctatgttcggtaaaatctacacccattgcttccttatct attaaacctaggccatgttcgacgaaatctacacacttccgtgagctgtt agatctacatggaattgctcattctggcacaaaacatcattttttttttc attagttgccttgcatgaccaggctgaactgagagcaagtgtgtttgagg ctatagaagaggaggaccgtgtgatagaggagaacgaagatggcgggaat cctgctttgcttggtagctgcaacgaccgtgctaagcagctgcatgcttc accgtcaggtaggttattaacagcacttataggtgaatacttggagtggg cccagctaagtcacacgatgaaagtttatttgccagaatgtaatctgccc aaggatttttggaagaatgagctaaaggatttctctaacaaaaatggagc tgaagggagcaggagcgctgagagtggtcccatgcttctagatgttcttg aaggatatctcaaatatgagaatttgtctcaaacgaggatggctggtagg agaatgatcaattctgaacctgagccaacattgaatactgagcatcggaa catgaggaggccaccatcttcatcttcaatcgctggcatgcctc

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