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GrainGenes Sequence Report: BQ470347

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Sequence
BQ470347
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC176316
NCBI UniGene
Hv.5277
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'Transcribed locus, moderately similar to NP_001055908.1 Os05g0491200 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HX
Tissue
apex
Developmental Stage
apex (3-5 mm in size)
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
HX02K16r HX Hordeum vulgare subsp. vulgare cDNA clone HX02K16 5-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold
vulgare
Clone
HX02K16
Probe
GBM1411
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 545 Std Error: 0.00; Plate: 2 row: K column: 16; Seq primer: M13rev.
Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.
DNA
ccatgattacattccagacagtcttgacagcgtggcggaattcgacgcgc
ccccatcgcctgaacacagctacagcgtggtgttcccagccgacgaggag
tttggcaaggagccgcccgctcttccaccacagcttctcatgtctgtcct
tggcggcaccgacaactccgacgagcacgctccgaaaccgaagccgcagc
acgtcgtcctcgaccatctgttcatcgagaaaggatgggggtcgcagtct
ctgctcgccctcggagtcacccaccggttccagtcgaagtatgtgaacgt
cgtgctctacaaaccgctgatgaggaggtgaccgggtgaccgatgccgct
ggtggaagatgaaaggaagcagcggcgtcgaactaggactgattgggggt
gaataagagagtgtagcttgacaatgtacataaggggagatcaggaacca
tctgtttctctctctttctttctttctttctttcttgagtgaactcttct
tgtggcatgaggtccgaacggtctgaactgaactccggtgaaatg

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