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GrainGenes Sequence Report: BQ469891

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Sequence
BQ469891
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC155714
NCBI UniGene
Hv.6655
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'APETALA2-like protein (AP2L1)'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HX
Tissue
apex
Developmental Stage
apex (3-5 mm in size)
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
HX01C17T HX Hordeum vulgare subsp. vulgare cDNA clone HX01C17 5-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold
vulgare
Clone
HX01C17
Probe
GBS800
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 672 Std Error: 0.00; Plate: 1 row: C column: 17; Seq primer: T3.
Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.
DNA
acaccgcgcacgcggccgcaagggcctacgatcgcgcggcgatcaagttc
cgggggctggaggccgacatcaacttcaatctgagcgactacgaggagga
tttgaagcagatgaggaactggaccaaggaggagttcgtgcatatcctcc
gccgccagagcaccgggttcgccagggggagctcgaagtaccgcggcgtc
acgctccacaagtgcggccgctgggaggcaaggatgggccaactgctcgg
caagaagtacatatatcttgggctgttcgacagcgaagttgaagctgcaa
gagcgtacgacagggcggcgattcgcttcaatgggagagatgctgtgact
aactttgatagtagctcctacaatggagatgctacacctgacgtcgaaaa
tgaggcaattgttgatgctgatgctcttgacttggatctaaggatgtcgc
aacctaccgcgcacgatcccaagagggacaacatcatcgccggccttcag
ttaacttttgattcccctgagtcgtcaaccacaatggtcttctctcagcc
aatgagctcatcgtcccagtggcctgtgcatcaacatggcacagcagtac
cacctcagcagcaccagcgtttgtacccatctgcttgtcatggcttctac
cccaacgtacaggtgcaggtgc

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