GrainGenes Sequence Report: BQ467551
Sequence
BQ467551
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC156530
NCBI UniGene
Hv.2956
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare UniGene title 'Transcribed locus, strongly similar to NP_001049628.1 Os03g0262400 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HS
Tissue
embryo + scutellum
Developmental Stage
0-16 hours after imbibition
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
HS04C07r HS Hordeum vulgare subsp. vulgare cDNA clone HS04C07 5-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold vulgare
Clone
HS04C07
Probe
GBR1885
Remark
DB_xref: taxon:112509 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 558 Std Error: 0.00; Plate: 4 row: C column: 7; Seq primer: M13rev. Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.
DNA
cggcacgaggctctctctctctgaactcccaacccagccgaccaaatcgc
cgcacgagggggcgccatggaggcggcggtggagaggaaggtgcccgagc
aggaggagcagctacagccgtcgcacgcccgggccgaggacgcgccgccc
gccgccgtcgaggaggaggacgaggcggaggccgaggagagcgagcgccg
caaccgcgagctcaaggccggcctccatcctctcaggcgcaagctcgtgc
tctggtacacacgccggacgcccgggacgaggtcgcagtcgtacgaggac
aacatcaagaagatcgtcgatttcagcactgtcgagtcgttctgggtttg
ctactgccacctcgcacgcccgtccgccctaccgagccccacggacctgc
atcttttcaaagagggtgtccgtccactctgggaggatcctgcaaatcgg
aatggcggcaagtggattatacggttcaagaaggctgtttctgggcgttt
ctgggaggatttggtgctggttctagtgggtgaccaacttgattatagtg
atgatgtt

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BQ467551
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