GrainGenes Sequence Report: BQ466906
Sequence
BQ466906
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC158272
NCBI UniGene
Hv.21396
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare UniGene title 'CDNA clone: FLbaf78h09, mRNA sequence'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HS
Tissue
embryo + scutellum
Developmental Stage
0-16 hours after imbibition
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
HS01P06T HS Hordeum vulgare subsp. vulgare cDNA clone HS01P06 5-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold vulgare
Clone
HS01P06
Probe
GBM1159
Remark
DB_xref: taxon:112509 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 648 Std Error: 0.00; Plate: 1 row: P column: 6; Seq primer: T3. Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.
DNA
cggcacgaggagcaaatctagctatctcatcatctccggcttagctcacc
accactactattgctagcttgtgatcgaaatggctcgcgctgcagctact
cagctcgtgctggtcgccatggtggccgctatgctcctcgtagccaccga
cgcggccatctcctgcggtcaggtgagctctgccttgagcccttgcatct
cctatgcccgcggcaacggcgccaaaccgcctgtggcctgctgcagcggc
gtcaagagactggccggcgcagcccagagcaccgctgacaagcaagcggc
gtgcaagtgcatcaagagcgctgcgggtgggctaaacgctggcaaggccg
ccggaatcccctccatgtgcggcgtcagcgtcccctacgccatcagcgct
tccgtcgactgctctaagattcgctgatcgaccacttgctgtcatccttg
ctgcctatagctccagctatcgatcctgagacactgagtacgttgaggtt
atttacacaacacacacacacatatatatgaataaatgctctcatatatt
atctccatgtgggagagaaagagagagagagaagtacgtacgttgagcca
gctctgcatggccggcacactgttgtatcgatgttataattaatattt
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BQ466906
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||