GrainGenes Sequence Report: BQ465617
Sequence
BQ465617
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC192271
NCBI UniGene
Hv.17020
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare UniGene title 'CDNA clone: FLbaf117n08, mRNA sequence'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HU
Tissue
germinating seeds
Developmental Stage
germinating seeds, 16-48 h
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
HU04C23r HU Hordeum vulgare subsp. vulgare cDNA clone HU04C23 5-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold vulgare
Clone
HU04C23
Probe
GBM1236
Remark
DB_xref: taxon:112509 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 537 Std Error: 0.00; Plate: 4 row: C column: 23; Seq primer: M13rev. Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); germinating seeds, 16-48h. Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable. Average insert size is 1.2 kb
DNA
cggctccgtcttccgcctcgggatagaacgagagcggccgctagccacac
ggcacagccctgtagaaagtcaatttgaggaaaaaccttgaccaaagtga
ccagatgtttaagcctagttggaccgtgtccttccaaatgaatcaaagtg
tgcttgaggccaagatatgactgtatatggtgaagattggctgcaactct
tctcccatgcgtacccaatctcatctctgagcatctcacgggtcacctcc
tcctgagttgactcaaccacaccaacaaaagagagagagagagagagaga
gagagagagagagagagagagagagattggtctgaggagagcagaaaaca
acaagaccaagagagaccgcgaatggaaccggaagaagcggcagcagcgc
aggcgaaaggggatctcctccgcaactttgagcgaatcctccatgacgac
cccctcatagacgaggtcggattcctgcatcccacgcagttccactcact
actcgtagacagcgccaacaaatctgcgttccaatac

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BQ465617
|
| |||||
|
| |||||
|
| |||||
|
| |||||
|
| |||||
|
| |||||
|
| |||||
|
| |||||
|
| |||||
|
| |||||
|
| |||||
|
| |||||
|
| |||||
|
| |||||
|
| |||||
|
| |||||
|
| |||||
|
|
![]()
![]() ![]() GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||