GrainGenes Sequence Report: BQ461301
Sequence
BQ461301
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC163489
NCBI UniGene
Hv.7146
DB Remark
Locus Source: Hordeum vulgare UniGene title 'CDNA clone: FLbaf16h11, mRNA sequence'
Keyword
EST
Species
Hordeum vulgare
Cultivar
Golden Promise
Clone Library
HD
Tissue
callus
Developmental Stage
callus (5-10 mm in diameter)
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
HD04L04r HD Hordeum vulgare cDNA clone HD04L04 5-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold
Clone
HD04L04
Probe
GBM1326
Remark
DB_xref: taxon:4513 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 436 Std Error: 0.00; Plate: 4 row: L column: 4; Seq primer: M13rev. Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 1 kb
DNA
cggcacgaggcagagcacaacccttggaggctgcttcttcttcttcttct
tcttcttccatatatagtaacccaccctaccctttgccaagatgtacgac
gctccgtcggggcaggagatgtcctacaccgaccacgtgcagaggcgcca
cgaggagaagggctgcctctacgcatgcgtgttcacggcgttgtgctgct
tctgctgctacgagacctgcgagtgctgcctcgactgcctctgctgctgc
tgcaactaaaccagccatggccgccatggacggaggccgcaaggcgcagc
agagacccccaccggcctcagccgcctttcataataagcatatatgtgtg
tctagtgtctcagctcgatcagaagaactgtatcagatatttctgtactg
cctttcacaataagcatatatatggtttctagtctt

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BQ461301
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