GrainGenes Sequence Report: BQ460543

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Sequence

BQ460543

External Databases

Data at GenBank

Data at EMBL

Data at DDBJ

DB Remark

Locus Source: Hordeum vulgare subsp. vulgare

Keyword

EST

Species

Hordeum vulgare subsp. vulgare

Cultivar

barke

Clone Library

Tissue

embryosac

Developmental Stage

0-7 DAP (days after pollination)

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

HA09D03r HA Hordeum vulgare subsp. vulgare cDNA clone HA09D03 5-PRIME, mRNA sequence.

Strain

lab_host XL10-Gold

vulgare

Clone

HA09D03

Probe

GBS853

Remark

DB_xref: taxon:112509

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 569 Std Error: 0.00; Plate: 9 row: D column: 3; Seq primer: M13rev.

Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); 0-7 DAP(days after pollination) Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable. Average insert size is 1 kb

DNA

cggcacgaggctctgcttgagatgatgatctgccacctcccctctccgtt
agaagcacaggaatatcgtgtggagaacctgtatgagggcccccttgatg
ataagtacgcagaagctattaaacaatgtgatcctgaaggtcctcttatg
ctgtacatttccaagatggttccagcatctgatgtgggcagatttttcgc
gtttggtcgtgtcttttctggaaggattgcagctggcatgaaggtccggg
tcatggggccttcctatgtccccagcatggaaactgatttgttcgtggag
tctgtccagcgtaccattatatggatgggaaagaatcaatgtgacgttcc
ggatgttccttgtggtaacacagttggactgattggtctggatgcatcta
taacaaagagtgctaccctgacaagtgagaaggaggttgatgcacaccca
atcagagcaatgaagctccctgtatgccctctgatgtttgttacagttaa
ctgcaaggttccgtttgagtctcccaaacttgtacaaggtttgaagcgtc
tggcgatgtctgatcccgt


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