GrainGenes Sequence Report: BQ295146
Sequence
BQ295146
Contig
Ta.23.1.S1_at NSFT03P2_Contig5225
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC304570
NCBI UniGene
Ta.23
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Voltage dependent anion channel (VDAC)'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Clone Library
Wheat unstressed root tip cDNA library
Tissue
Root tip at 1.0 to 1.5 mm stage
Developmental Stage
Four-day old seedling
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE2858_G03_N06ZS Wheat unstressed root tip cDNA library Triticum aestivum cDNA clone WHE2858_G03_N06, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE2858_G03_N06
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown under hydroponic conditions for four days. Root tips were excised and snap frozen (Ross and Gustafson) and total RNA was prepared at University of Missouri, Columbia. Poly(A) RNA was purified, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript SK(- )phagemids in the TJ Close lab (Chin and Close) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown under hydroponic conditions for four days. Root tips were excised and snap frozen (Ross and Gustafson) and total RNA was prepared at University of Missouri, Columbia. Poly(A) RNA was purified, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript SK(-)phagemids in the TJ Close lab (Chin and Close) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
gagccgcgatctctctcccgctctcttctccccaccaccacccgagtcgt
cgtctccgtcgccggccgccatggctccgggcctctacaccgacatcggc
aagaagaccagagatctgctctacagggactactgcacgcaccagaagtt
caccctcaccacatgcacccccgagggcgtcgcaatcacagctgcaggaa
ctaggaaaaacgagtcaatctttggcgagcttcagacccagcttaagaac
aagaacttgacagttgatatcaaagcaaactcagagtcagatcttctgac
aacattcacagttgatgagtttgcaactccagggctgaaatcaattctca
gcttggttgttccagaccagaggtcagggaagcttgaactccagtatctc
catgaattcgccggtgtcaatgcaagcgttgggctgaaccccaaccctat
ggttaacctttctggtgtattcggaagcaaagaactgtcagttggtgttg
acgtttcatttgacacggcaactagcaatttcaccaagtacaacgctgct
ttaagcctcacgtatccggatcttattgcttcactccacctgaacaatca
cggtgacaccttgactgcgtcttactaccacttagtaaagtcacattcaa
gcactgc
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BQ295146
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||