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GrainGenes Sequence Report: Ta.13157.1.S1_at

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Sequence	BQ295074
Contig	Ta.13157.1.S1_at
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC304891
NCBI UniGene	Ta.13157
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus'
Keyword	EST
Species	Triticum aestivum
Cultivar	Chinese Spring
Clone Library	Wheat unstressed root tip cDNA library
Tissue	Root tip at 1.0 to 1.5 mm stage
Developmental Stage	Four-day old seedling
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE2858_A01_B02ZS Wheat unstressed root tip cDNA library Triticum aestivum cDNA clone WHE2858_A01_B02, mRNA sequence.
Strain	lab_host E. coli SOLR
Clone	WHE2858_A01_B02
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown under hydroponic conditions for four days. Root tips were excised and snap frozen (Ross and Gustafson) and total RNA was prepared at University of Missouri, Columbia. Poly(A) RNA was purified, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript SK(- )phagemids in the TJ Close lab (Chin and Close) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown under hydroponic conditions for four days. Root tips were excised and snap frozen (Ross and Gustafson) and total RNA was prepared at University of Missouri, Columbia. Poly(A) RNA was purified, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript SK(-)phagemids in the TJ Close lab (Chin and Close) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA	cggcacgaggcaaagccgagggtacctgctgcagtagcaatagcacatca tgcacttgtgactgcaacaccatagctcaagaacgttgcagcagcctcac cgacaactacacagattgccaagcttgcactaacagcatattcaatcagt gcaataccacctgtaacgatgactgcaacaacaactgcaagaagaaaggg tgtcatgcataggaatagcatgactaatgatggttgtgcttgtacctacg tttgtgtttgatcttttttatataaacgtgcgccatgacttggttttatg gggtggcaccggagatatgggttgtggcgcatcacctccagtgttttagt tggggatttgcattttgtttatttatactgaataataatattgaacggaa gaaatttcaccaagttanaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa

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