GrainGenes Sequence Report: BQ294783
Sequence
BQ294783
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC308158
wEST map position
BQ294783
NCBI UniGene
Ta.35953
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, strongly similar to NP_001067159.1 Os12g0586600 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
5AS 5BS 5DS
Clone Library
Wheat unstressed root tip cDNA library
Tissue
Root tip at 1.0 to 1.5 mm stage
Developmental Stage
Four-day old seedling
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE2854_C12_F24ZS Wheat unstressed root tip cDNA library Triticum aestivum cDNA clone WHE2854_C12_F24, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE2854_C12_F24
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown under hydroponic conditions for four days. Root tips were excised and snap frozen (Ross and Gustafson) and total RNA was prepared at University of Missouri, Columbia. Poly(A) RNA was purified, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript SK(- )phagemids in the TJ Close lab (Chin and Close) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown under hydroponic conditions for four days. Root tips were excised and snap frozen (Ross and Gustafson) and total RNA was prepared at University of Missouri, Columbia. Poly(A) RNA was purified, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript SK(-)phagemids in the TJ Close lab (Chin and Close) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
TaAffx.50789.1.S1_at 224 WHE2AFFY
[ Show all 7 ]
DNA
gaggagacaggtgctgttacccctcttgataaagaactgctggcaagctc
aatggtattattgatggttttgctaatgaagctcttaggacattgtgcct
tgcttacagggaaatggaagaaggcttttccattgaagagcaattaccac
agcaagggtacacatgcattgctatcgtaggtattaaagatcctgttcgc
ccaggtgtgagagagtctgttgcaatttgccgctctgctggagttacggt
gagaatggtcacaggtgacaacataaatacagcaaaggcgattgcccgtg
aatgtggtatactcaccgaagatggcctggccatcgagggaccggatttc
agggagaaaactcttgaggaactccttgtgcttgttccaaaaattcaggt
aatggcccgatcatcaccgctggataagcatacacttgtaaagcatttgc
gcacaacattcaatgaagttgttgctgttactggtgacggcacaaatgat
gctcctgctctgcatgaagcagatattggacttgcaatgggcattgccgg
gactgaggtggcgaaagagagtgccgatgtcatcattctggacgacaatt
tctctacaattgtaactgttgccagatgggggcgctctgtttacgtcaac
attcagaaatttgtgcagtttc
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BQ294783
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||