GrainGenes Sequence Report: BQ281029
Sequence
BQ281029
Contig
Ta.6842.2.S1_at NSFT03P2_Contig14306
External Databases
Data at GenBank Data at EMBL Data at DDBJ
DB Remark
Locus Source: Triticum aestivum (bread wheat)
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Clone Library
Wheat unstressed seedling shoot normalized cDNA library
Tissue
Etiolated shoot
Developmental Stage
Five day old seedling
Data Source
genbank Release 135, Apr 15 2003
Title
WHE3007_F03_L05ZS Wheat unstressed seedling shoot normalized cDNA library Triticum aestivum cDNA clone WHE3007_F03_L05, mRNA sequence.
Strain
lab_host E. coli DH10B
Clone
WHE3007_F03_L05
Remark
DB_xref: taxon:4565 Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid pBluescript SK; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Roots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. The cDNA clones were in vivo excised to give pBluescript phagemids before normalization was carried out. The mass excision of phagemid library and normalization were done in HT Nguyen lab by D. Zhang at Texas Tech Univeristy. Normalization protocol used was that of Soares'. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
ttaattgaaatcattcaagcacaaggtgatcttgatgcgcagtttgatgt
ttctgatatcgatcgtatagagaggtggacctcatgcatctatatggctc
ttcctatcttcacgagaggtgcatcaaggaacaagtttctcaactatttt
gctaagcaaatcgttccagttttcgataagattccggaggagaagaaact
ggatttacttaagacagttgctgctagctcgccatatgctgtagcacaag
attcacgccagctgcttccatccgttgttcaattgctcaagaagtatatg
cctgggaagaaggtagatgatattaatcacaattatgtcgagtgcttgct
atacacttttcaccatttggctcataagacaccaaacactacaaacagtc
tctgtggttacaagatcgttactggacagccatcagatagacttggagag
gatttctcagaacattacaaagattttattgagaggttaactggaacgga
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BQ281029
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||