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GrainGenes Sequence Report: BQ246913

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Sequence
BQ246913
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
DB Remark
Locus Source: Triticum aestivum (bread wheat)
Keyword
EST
Species
Triticum aestivum
Cultivar
Glenlea
Clone Library
TaE15
Tissue
developing seeds
Developmental Stage
15 days after anthesis
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
TaE15003G02R TaE15 Triticum aestivum cDNA clone TaE15003G02R, mRNA sequence.
Strain
lab_host E. coli DH10B
Clone
TaE15003G02R
Probe
MAG460
MAG1592
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Dr. Sylvie Cloutier; Cereal Research Centre, Agriculture and Agri-food Canada; 195 Dafoe Rd, Winnipeg, MB, Canada R3T 2M9; Tel: (204) 983-2340; Fax: (204) 983-4604; Email: scloutier@em.agr.ca; was cloned directionally, not all sequences generated with reverse; primer were from the 5' end (same with forward primer and 3' end).; Average insert size is >1.4 kb; Plate: 003 row: G column: 02; Seq primer: M13 Reverse.
Locus Comment: Contact: Dr. Sylvie Cloutier; Cereal Research Centre, Agriculture and Agri-food Canada; 195 Dafoe Rd, Winnipeg, MB, Canada R3T 2M9; Tel: (204) 983-2340; Fax: (204) 983-4604; Email: scloutier@agr.gc.ca; was cloned directionally, not all sequences generated with reverse; primer were from the 5' end (same with forward primer and 3' end).; Average insert size is >1.4 kb; Plate: 003 row: G column: 02; Seq primer: M13 Reverse.
Note: Vector: pCMV-SPORT6.0 (Invitrogen Technologies); Site_1: NotI; Site_2: MluI; mRNA obtained from wheat seeds of cultivar Glenlea 15 days post-anthesis
DNA
ctggaatgaagaacagcgaaagtgggaggatagcctgagggcaacgttgt
gttccgtcggcggttccggaatcgttataatcgtcacgactcggagccca
cgagcagcgtctataatgggcacgcttcaaacccatgagctggcacatct
gagtgaacaacactcatgggaattgttctcaaagaaagcatttaagggaa
atgtgcgagagcaggcagagttgatgtcgatcggcaagcgtatcgtcagc
aaatgcaaggggcaccctcttgctctgagtgcaatggggggtttgatgtg
ttcaaaaggacgggcctgggaatgggaggccattgcagaaagcaatattg
gggatagtgttgcaggcaaacaagaaatcatatccatactcaaattgagc
taccaacacttatcctctgaaatgaagcaatgctttgccttctgcgccgt
cttctacaagaacagtgagatggaaaaaagcatgttgatccaactatgga
tggccaatgggtttattcaagaagagggaatgatggat

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