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GrainGenes Sequence Report: BQ238952

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Sequence
BQ238952
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC279524
NCBI UniGene
Ta.48607
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Serine/threonine protein kinase 1'
Keyword
EST
Species
Triticum aestivum
Cultivar
Glenlea
Clone Library
TaE05
Tissue
developing seeds
Developmental Stage
5 days after anthesis
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
TaE05039C02R TaE05 Triticum aestivum cDNA clone TaE05039C02R, mRNA sequence.
Strain
lab_host E. coli DH10B
Clone
TaE05039C02R
Probe
MAG1753
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Dr. Sylvie Cloutier; Cereal Research Centre, Agriculture and Agri-food Canada; 195 Dafoe Rd, Winnipeg, MB, Canada R3T 2M9; Tel: (204) 983-2340; Fax: (204) 983-4604; Email: scloutier@em.agr.ca; was cloned directionally, not all sequences generated with reverse; primer were from the 5' end (same with forward primer and 3' end).; Average insert size is >2.0 kb; Plate: 039 row: C column: 02; Seq primer: M13 Reverse.
Locus Comment: Contact: Dr. Sylvie Cloutier; Cereal Research Centre, Agriculture and Agri-food Canada; 195 Dafoe Rd, Winnipeg, MB, Canada R3T 2M9; Tel: (204) 983-2340; Fax: (204) 983-4604; Email: scloutier@agr.gc.ca; was cloned directionally, not all sequences generated with reverse; primer were from the 5' end (same with forward primer and 3' end).; Average insert size is >2.0 kb; Plate: 039 row: C column: 02; Seq primer: M13 Reverse.
Note: Vector: pSPORT-P (Invitrogen Technologies); Site_1: NotI; Site_2: MluI; mRNA obtained from wheat seeds of cultivar Glenlea 5 days post-anthesis
DNA
ccacgcgtccgcgcctcgcagtcaggcaggcgcagcacgccaatccgccc
ccggattccccccgccgtccggccgccgccgccgctccccccctcctccg
gccgccgccccgctccccgccgagggaccattttattcctggacaacaga
tcattttcagaagctttgccattggtgtgtgttgcatctttgaaagatga
ttgagggggcaaggttccacaatatgctgggcggcgctggcatcggcggc
ggcagagggaagctggagaacgagagcaacggcttctacgacatgccgta
ttaccacaaggtcggggagagctcccacatgtcggtggacagcgcggaca
acatgaactcgatgagctatgtcggcggctcggtcgccatgtcggtggac
aacagcagcgtggcctccaacgagtcccgcaccgtcatgctcaaccaccc
aggcctccgtgatgtccccacaccaaactattcggtttgcaacagtgtca
tctaccccaacaaggctgccgcgtctgttctcaaggaggatgctctggct
cgggttttgatggacccgactcatccaacagagatactcactagctatga
ggcgtggaccattgatctggggaagctggacatgggggctccttttgctc
agggggcctttgggaagctgtatc

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