GrainGenes Sequence Report: BQ172211
Sequence
BQ172211
Contig
Ta.4599.1.S1_at NSFT03P2_Contig8103
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC281392
wEST map position
BQ172211
NCBI UniGene
Ta.48399
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Clone wr1.pk0067.h2:fis, full insert mRNA sequence'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
1AS 1BS 1DS
Clone Library
Chinese Spring wheat drought stressed leaf cDNA library
Tissue
Leaf
Developmental Stage
Full tillering stage
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE2013_A06_A11ZT Chinese Spring wheat drought stressed leaf cDNA library Triticum aestivum cDNA clone WHE2013_A06_A11, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE2013_A06_A11
Probe
WHE2013_A06_A11
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were given a gradual stress down to 80%, 70% and to 60% RWC at Texas Tech University (D. Zhang in HT Nguyen lab). Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab at the University of California, Riverside (Akhunov, Chin, Choi, Close, Fenton , Kianian, Otto, Simons, Zhang). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were given a gradual stress down to 80%, 70% and to 60% RWC at Texas Tech University (D. Zhang in HT Nguyen lab). Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab at the University of California, Riverside (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons, Zhang). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
ttttttttttttttttttttttttttaatccaaaaacaagcctgatactt
tttttaagaatcacaacaagccaagacacgagacatatcaaatgatatca
gcatgcgtatatatatcaatctaaccggacggccaacacttcttcaaaat
cgctaacaaagtgtagaattgacgttcttatatctcaaggcagacatctg
cagcccttcaaagatcggccctcccagggtatcttgggaatgggatgaca
tctctgatgttctccaggccggtggcgaaaaggatcatcctctcgaaccc
taggccgaacccgctgtgcttcactgagccaaagcgtcggaggtccaggt
accactcatatggctcc
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BQ172211
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||