Query (optional)   in Class  

GrainGenes Sequence Report: Ta.12270.1.S1_at

[Submit comment/correction]

Sequence
BQ171984
Contig
Ta.12270.1.S1_at
NSFT03P2_Contig14337
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC322910
NCBI UniGene
Ta.12270
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, strongly similar to NP_001063817.1 Os09g0541900 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Clone Library
Wheat heat stressed spike cDNA library
Tissue
Whole spike
Developmental Stage
Spikes at 5, 10, 15 and 20 days after anthesis
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE1710_A09_A18ZT Wheat heat stressed spike cDNA library Triticum aestivum cDNA clone WHE1710_A09_A18, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE1710_A09_A18
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Spikes at 5, 10, 15 and 20 days after anthesis were heat stressed under two conditions at Texas Tech University (D. Zhang in HT Nguyrn lab): (1) at 38 C for 4 hours and (2) 5 days of cyclic treatment of 38 C for 4 hours. Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton, Malatrasi) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
tttttttttttttttttttagtcctcaatcagcttcccacaaagcaaaca
caggatgcagtgcaaaacagtttccgacaagctctctcagttcctcgatt
acagtatctcatggaaaaataaggcacttcctaccaatttgtagtgatat
aagaatttgcagtgaaaaataaggtgcttcctacgaacataaaaaaccgc
accgagtaatcattcagattgtccctgcaaggatattggactgcggcccc
tgaaattgacagagtagtaccagcttcttcgtaaagaacaggcagcacct
gaaagcatggagagggaatctagaagtcatcatcgtcctcctcggccatg
tgttttgcaagttcttcctcctgctggagtctctcacgcctcttctctgc
actctccttttccttgtgagaa

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.