GrainGenes Sequence Report: Ta.12582.1.S1_at
Sequence
BQ171814
Contig
Ta.12582.1.S1_at NSFT03P2_Contig16065
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC310441
wEST map position
BQ171814
NCBI UniGene
Ta.12582
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Clone wkm1c.pk005.m16:fis, full insert mRNA sequence'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
3AS 3BS 3DS
Clone Library
Wheat heat stressed flag leaf cDNA library
Tissue
Flag leaf
Developmental Stage
Full tillering stage
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE1663-1666_E08_E08ZT Wheat heat stressed flag leaf cDNA library Triticum aestivum cDNA clone WHE1663-1666_E08_E08, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE1663-1666_E08_E08
Probe
WHE1663-1666_E08_E08
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants at full-tillering stage were heat stressed under four conditions: (1) 38 C for 2 hours, (2) 38 C for 4 hours, (3) 2 days of cyclic treatment of 38 C for 2 hours, (4) 2 days of cyclic treatment of 38 C for 4 hours, at Texas Tech University (D Zhang in Nguyen lab). Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton, Malatrasi) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
tttttttttttttttttttttttatgaaatatctgctggagaagactaga
aagttttcagcatcattccagatttatgaagatcatgttccgacaacact
aggaatacagtgaatactcgttaaaaatacgcgctctgcaggttaaacaa
acagcatgaacaaatgctcggttacaaaacaaaaagctaaacgagagcat
tgccttctcatctcattaaagttgtggaaaaaacaaagatcaatctccac
atgacagtgacgcccagtattgctggccaccagaactccacataaacagc
cgctggcagcaagtgatatactccctggatatatcagtgaaaacgacgga
agctaacttctggtcaagcgtcttcttcaatcatgattcagatcaagcgt
tgctcttctggtcttcgattttcttctggaacgatgtgaaccattgcttc
caagccgcgtctccttgttgctcgatccaagacaagaaacagctgtctaa
caggcagaacaagttcacgtacagcagttgatatcgcacgggaatgaagc
gaaagtttgcgatctgcacagccggccagatggctccccctaaaaccaga
gcaggaatgatatccctcttcacatcatccttcacctgct
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: Ta.12582.1.S1_at
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||