GrainGenes Sequence Report: NSFT03P2_Contig7537
Sequence
BQ171700
Contig
Ta.7036.1.A1_at NSFT03P2_Contig7537
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC341979
wEST map position
BQ171700
NCBI UniGene
Ta.9228
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, weakly similar to NP_001067535.1 Os11g0225000 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
7AL 7BL 7DL
Clone Library
Wheat heat stressed flag leaf cDNA library
Tissue
Flag leaf
Developmental Stage
Full tillering stage
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE1655-1658_O03_O03ZT Wheat heat stressed flag leaf cDNA library Triticum aestivum cDNA clone WHE1655-1658_O03_O03, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE1655-1658_O03_O03
Probe
MAG1708 WHE1655-1658_O03_O03
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants at full-tillering stage were heat stressed under four conditions: (1) 38 C for 2 hours, (2) 38 C for 4 hours, (3) 2 days of cyclic treatment of 38 C for 2 hours, (4) 2 days of cyclic treatment of 38 C for 4 hours, at Texas Tech University (D Zhang in Nguyen lab). Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton, Malatrasi) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
tttttttttttttttttttgcttcaatttcttgttgcaacccatcttgat
caataataaaaagatatagtttcacaaaataaagaaatttctacgtttcc
agactggcatatatagcatcgaatggtcttatttccaaagcaaattttga
gtcttccagcaataataacacacgttttatttcttcttgaaacctcctag
aaaacccatcttcttgctaaccggagatgcatgagggaccacgtttgcca
tttcatttgcacgtagtccgttgagcgtgtcaacaatttcttctatggga
ggcctgactcgccgatcaatctcagcacattttagcccaacttcaatgca
tatttaacttgatggaggccttctgattctaaggatgcatatttggacgc
tatgtgctccagtgtccaatcttcgcgtactttatcaataaagtttcttg
ctgagggttggtttgtctttgcagttctgctctccagtactgatctcgag
tattaataagccaaaactatatatgtctgact
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: NSFT03P2_Contig7537
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||